Perfection in PAINT - super-resolution microscopy with DNA-PAINT kits

With Massive Photonics Neuro-Imaging Kits, you can stain up to six targets simultaneously, achieving visualization of cytoskeleton-associated and synaptic proteins with resolutions below 20 nm. Immerse yourself in the world of high-resolution multiplexing!

Our partner company Massive Photonics offers ready-to-use kits for multi-target staining of various neuronal targets using some of our well-established antibodies for DNA-PAINT (DNA-Point Accumulation for Imaging in Nanoscale Topography). This localization based super-resolution microscopy method employs short, dye-labeled single stranded DNA that transiently binds its complementary sequences, which are attached to target-specific antibodies or nanobodies.

• Imager strand – a dye-labeled DNA oligo present in imaging buffer
• Docking strand – a DNA oligo complementary to the imager strand that is delivered to the target of interest via binders (e.g. antibodies, single domain antibodies)

When a dye-labeled imager transiently hybridizes to a docking strand in DNA-PAINT imaging buffer, fluorescent signal or “blinking” occurs. To create a super-resolution image, a camera captures blinking over a period of time (10 – 50 min) and the resulting image is post-processed using the Picasso data analysis software – a free software developed for DNA-PAINT image analysis.

Why use DNA-PAINT?

• Ultra resolution! Resolve 10 – 15 nm distances in cells.
• Unlimited target multiplexing! In DNA-PAINT, spectrally compatible dyes aren’t needed to image multiple targets at a time. Instead, you can simply couple different docking strands (e.g. different DNA sequences) to your target binders and image each target sequentially using the same laser line with its corresponding imager strand.
• Target Counting! The predictable nature of DNA binding kinetics allows you to quantify how many proteins are present in cells.

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