Cat. No.: 214-0P
Amount: 100 µg
Price:
$110.00
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| Cat. No. 214-0P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 214 002, 214 003, 214 004, 214 005, 214 006, 214 011, 214 011BT, 214 011C3, 214 011C5, 214 008, 214 318, 214 009 |
| Remarks |
This control protein consists of the recombinant protein (aa 3 - 251 of human calbindin D28k) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 214-0p.pdf |
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Calbindin D28k (CALB1, D28K or CAB27) and Calretinin (Calbindin D29k) are members of the EF-hand calcium-binding protein family, playing essential roles in intracellular calcium homeostasis, neuroprotection, and signal transduction (1). Calbindin D28k is widely expressed in tissues that experience high calcium influx, such as the brain, sensory organs, endocrine tissues, and the enteric nervous system (ENS). In the central nervous system (CNS), Calbindin D28k is an important marker for specific neuronal populations, particularly in Purkinje cells of the cerebellum and GABAergic and glutamatergic neurons of the cortex (2). Outside the CNS, Calbindin D28k plays crucial roles in non-neuronal tissues. In the retina, it contributes to photoreceptor function and calcium signaling (3). In the pancreas, it is present in α- and β-cells (4). Additionally, it was reported that 31% of myenteric neurons and up to 95% of submucosal neurons in the human ENS express calbindin, suggesting its involvement in both motor and secretory functions (5).