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Home > Products > 214 004

Calbindin D28k antibody - 214 004

Calbindin D28k is a vitamin D-dependent calcium binding protein
Guinea pig polyclonal antiserum
Cat. No.: 214 004
Amount: 100 µl
Price: $395.00
Cat. No. 214 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Full-length recombinant human Calbindin D28k protein (UniProt Id: P05937)
Reactivity Reacts with: human (P05937), rat (P07171), mouse (P12658), monkey, ape, cow.
Other species not tested yet.
Matching control protein/peptide 214-0P
Data sheet 214_004.pdf

References for Calbindin D28k - 214 004

Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 214 004 ICC; tested species: mouse
The Bacterial Enzyme RfxCas13d Is Less Neurotoxic Than PspCas13b and Could Be a Promising RNA Editing and Interference Tool in the Nervous System.
Wu QW, Kapfhammer JP
Brain sciences (2021) 118: . 214 004 ICC; tested species: mouse
Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 214 004 ICC; tested species: rat
Overexpression of the autism candidate gene Cyfip1 pathologically enhances olivo-cerebellar signaling in mice.
Busch SE, Simmons DH, Gama E, Du X, Longo F, Gomez CM, Klann E, Hansel C
Frontiers in cellular neuroscience (2023) 17: 1219270. 214 004 IHC; tested species: mouse
Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells.
Tsujimoto H, Hoshina A, Mae SI, Araoka T, Changting W, Ijiri Y, Nakajima-Koyama M, Sakurai S, Okita K, Mizuta K, Niwa A, et al.
Cell reports (2023) : 113602. 214 004 IHC; tested species: mouse
Decreasing mutant ATXN1 nuclear localization improves a spectrum of SCA1-like phenotypes and brain region transcriptomic profiles.
Handler HP, Duvick L, Mitchell JS, Cvetanovic M, Reighard M, Soles A, Mather KB, Rainwater O, Serres S, Nichols-Meade T, Coffin SL, et al.
Neuron (2022) : . 214 004 IHC; tested species: mouse
Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 004 IHC; tested species: mouse
Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.
Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, et al.
Cell reports (2021) 3611: 109704. 214 004 IHC; tested species: mouse
A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, Nasu M, et al.
Cell reports (2020) 311: 107476. 214 004 IHC; tested species: mouse
TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons.
Bowie E, Goetz SC
eLife (2020) 9: . 214 004 IHC; tested species: mouse
Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 004 IHC; tested species: mouse
Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 004 IHC; tested species: rat
Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress.
Alpár A, Zahola P, Hanics J, Hevesi Z, Korchynska S, Benevento M, Pifl C, Zachar G, Perugini J, Severi I, Leitgeb P, et al.
The EMBO journal (2018) : . 214 004 IHC; tested species: mouse
Timely regulated sorting from early to late endosomes is required to maintain cerebellar long-term depression.
Kim T, Yamamoto Y, Tanaka-Yamamoto K
Nature communications (2017) 81: 401. 214 004 IHC; tested species: mouse
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 004 IHC
Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 214 004 IHC; tested species: mouse
Prolonged Type 1 Metabotropic Glutamate Receptor Dependent Synaptic Signaling Contributes to Spino-Cerebellar Ataxia Type 1.
Power EM, Morales A, Empson RM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3618: 4910-6. 214 004 IHC; tested species: mouse
Synaptic Organization of Perisomatic GABAergic Inputs onto the Principal Cells of the Mouse Basolateral Amygdala.
Vereczki VK, Veres JM, Müller K, Nagy GA, Rácz B, Barsy B, Hájos N
Frontiers in neuroanatomy (2016) 10: 20. 214 004 IHC; tested species: mouse
High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease.
Bouvier DS, Jones EV, Quesseveur G, Davoli MA, A Ferreira T, Quirion R, Mechawar N, Murai KK
Scientific reports (2016) 6: 24544. 214 004 IHC; tested species: human
Functional and Physical Interaction of Diacylglycerol Kinase ζ with Protein Kinase Cα Is Required for Cerebellar Long-Term Depression.
Lee D, Yamamoto Y, Kim E, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3546: 15453-65. 214 004 IHC
An excitatory GABA loop operating in vivo.
Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I
Frontiers in cellular neuroscience (2015) 9: 275. 214 004 IHC
Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 004 IHC
Raf kinase inhibitory protein is required for cerebellar long-term synaptic depression by mediating PKC-dependent MAPK activation.
Yamamoto Y, Lee D, Kim Y, Lee B, Seo C, Kawasaki H, Kuroda S, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3241: 14254-64. 214 004 IHC
Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina.
Soto F, Ma X, Cecil JL, Vo BQ, Culican SM, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5426-39. 214 004 IHC
Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
Jurk D, Wang C, Miwa S, Maddick M, Korolchuk V, Tsolou A, Gonos ES, Thrasivoulou C, Saffrey MJ, Cameron K, von Zglinicki T, et al.
Aging cell (2012) 116: 996-1004. 214 004 IHC
Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs.
Alpár A, Künzle H, Gärtner U, Popkova Y, Bauer U, Grosche J, Reichenbach A, Härtig W
Brain research (2010) 1330: 9-19. 214 004 IHC
Excitation of Diverse Classes of Cholecystokinin Interneurons in the Basal Amygdala Facilitates Fear Extinction.
Rovira-Esteban L, Gunduz-Cinar O, Bukalo O, Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, et al.
eNeuro () 66: . 214 004 IHC; tested species: mouse
Single cell RNA sequencing uncovers cellular developmental sequences and novel potential intercellular communications in embryonic kidney.
Matsui I, Matsumoto A, Inoue K, Katsuma Y, Yasuda S, Shimada K, Sakaguchi Y, Mizui M, Kaimori JY, Takabatake Y, Isaka Y, et al.
Scientific reports (2021) 111: 73. 214 004 IHC-P; tested species: mouse
Cat. No.: 214 004
Amount: 100 µl
Price: $395.00
Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.
Alexander CJ, Barzik M, Fujiwara I, Remmert K, Wang YX, Petralia RS, Friedman TB, Hammer JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 351: e21092. 214 004 ICC; tested species: mouse
The Bacterial Enzyme RfxCas13d Is Less Neurotoxic Than PspCas13b and Could Be a Promising RNA Editing and Interference Tool in the Nervous System.
Wu QW, Kapfhammer JP
Brain sciences (2021) 118: . 214 004 ICC; tested species: mouse
Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 214 004 ICC; tested species: rat
Overexpression of the autism candidate gene Cyfip1 pathologically enhances olivo-cerebellar signaling in mice.
Busch SE, Simmons DH, Gama E, Du X, Longo F, Gomez CM, Klann E, Hansel C
Frontiers in cellular neuroscience (2023) 17: 1219270. 214 004 IHC; tested species: mouse
Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells.
Tsujimoto H, Hoshina A, Mae SI, Araoka T, Changting W, Ijiri Y, Nakajima-Koyama M, Sakurai S, Okita K, Mizuta K, Niwa A, et al.
Cell reports (2023) : 113602. 214 004 IHC; tested species: mouse
Decreasing mutant ATXN1 nuclear localization improves a spectrum of SCA1-like phenotypes and brain region transcriptomic profiles.
Handler HP, Duvick L, Mitchell JS, Cvetanovic M, Reighard M, Soles A, Mather KB, Rainwater O, Serres S, Nichols-Meade T, Coffin SL, et al.
Neuron (2022) : . 214 004 IHC; tested species: mouse
Total Number and Ratio of GABAergic Neuron Types in the Mouse Lateral and Basal Amygdala.
Vereczki VK, Müller K, Krizsán É, Máté Z, Fekete Z, Rovira-Esteban L, Veres JM, Erdélyi F, Hájos N
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 4121: 4575-4595. 214 004 IHC; tested species: mouse
Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.
Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, et al.
Cell reports (2021) 3611: 109704. 214 004 IHC; tested species: mouse
A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, Nasu M, et al.
Cell reports (2020) 311: 107476. 214 004 IHC; tested species: mouse
TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons.
Bowie E, Goetz SC
eLife (2020) 9: . 214 004 IHC; tested species: mouse
Defining the Vulnerability Window of Anesthesia-Induced Neuroapoptosis in Developing Dentate Gyrus Granule Cells - A Transgenic Approach Utilizing POMC-EGFP Mice.
Wei K, Chen P, Shen FY, Zhang Y, Liu YH, Wang ZR, Loepke AW, Wang YW, Deng M
Neuroscience (2019) : . 214 004 IHC; tested species: mouse
Non-canonical heterogeneous cellular distribution and co-localization of CaMKIIα and CaMKIIβ in the spinal superficial dorsal horn.
Larsson M
Brain structure & function (2018) 2233: 1437-1457. 214 004 IHC; tested species: rat
Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress.
Alpár A, Zahola P, Hanics J, Hevesi Z, Korchynska S, Benevento M, Pifl C, Zachar G, Perugini J, Severi I, Leitgeb P, et al.
The EMBO journal (2018) : . 214 004 IHC; tested species: mouse
Timely regulated sorting from early to late endosomes is required to maintain cerebellar long-term depression.
Kim T, Yamamoto Y, Tanaka-Yamamoto K
Nature communications (2017) 81: 401. 214 004 IHC; tested species: mouse
Abolished perineuronal nets and altered parvalbumin-immunoreactivity in the nucleus reticularis thalami of wildtype and 3xTg mice after experimental stroke.
Härtig W, Appel S, Suttkus A, Grosche J, Michalski D
Neuroscience (2016) 337: 66-87. 214 004 IHC
Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 214 004 IHC; tested species: mouse
Prolonged Type 1 Metabotropic Glutamate Receptor Dependent Synaptic Signaling Contributes to Spino-Cerebellar Ataxia Type 1.
Power EM, Morales A, Empson RM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3618: 4910-6. 214 004 IHC; tested species: mouse
Synaptic Organization of Perisomatic GABAergic Inputs onto the Principal Cells of the Mouse Basolateral Amygdala.
Vereczki VK, Veres JM, Müller K, Nagy GA, Rácz B, Barsy B, Hájos N
Frontiers in neuroanatomy (2016) 10: 20. 214 004 IHC; tested species: mouse
High Resolution Dissection of Reactive Glial Nets in Alzheimer's Disease.
Bouvier DS, Jones EV, Quesseveur G, Davoli MA, A Ferreira T, Quirion R, Mechawar N, Murai KK
Scientific reports (2016) 6: 24544. 214 004 IHC; tested species: human
Functional and Physical Interaction of Diacylglycerol Kinase ζ with Protein Kinase Cα Is Required for Cerebellar Long-Term Depression.
Lee D, Yamamoto Y, Kim E, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3546: 15453-65. 214 004 IHC
An excitatory GABA loop operating in vivo.
Astorga G, Bao J, Marty A, Augustine GJ, Franconville R, Jalil A, Bradley J, Llano I
Frontiers in cellular neuroscience (2015) 9: 275. 214 004 IHC
Cell age-specific vulnerability of neurons to anesthetic toxicity.
Hofacer RD, Deng M, Ward CG, Joseph B, Hughes EA, Jiang C, Danzer SC, Loepke AW
Annals of neurology (2013) 736: 695-704. 214 004 IHC
Raf kinase inhibitory protein is required for cerebellar long-term synaptic depression by mediating PKC-dependent MAPK activation.
Yamamoto Y, Lee D, Kim Y, Lee B, Seo C, Kawasaki H, Kuroda S, Tanaka-Yamamoto K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3241: 14254-64. 214 004 IHC
Spontaneous activity promotes synapse formation in a cell-type-dependent manner in the developing retina.
Soto F, Ma X, Cecil JL, Vo BQ, Culican SM, Kerschensteiner D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5426-39. 214 004 IHC
Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.
Jurk D, Wang C, Miwa S, Maddick M, Korolchuk V, Tsolou A, Gonos ES, Thrasivoulou C, Saffrey MJ, Cameron K, von Zglinicki T, et al.
Aging cell (2012) 116: 996-1004. 214 004 IHC
Slow age-dependent decline of doublecortin expression and BrdU labeling in the forebrain from lesser hedgehog tenrecs.
Alpár A, Künzle H, Gärtner U, Popkova Y, Bauer U, Grosche J, Reichenbach A, Härtig W
Brain research (2010) 1330: 9-19. 214 004 IHC
Excitation of Diverse Classes of Cholecystokinin Interneurons in the Basal Amygdala Facilitates Fear Extinction.
Rovira-Esteban L, Gunduz-Cinar O, Bukalo O, Limoges A, Brockway E, Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, et al.
eNeuro () 66: . 214 004 IHC; tested species: mouse
Single cell RNA sequencing uncovers cellular developmental sequences and novel potential intercellular communications in embryonic kidney.
Matsui I, Matsumoto A, Inoue K, Katsuma Y, Yasuda S, Shimada K, Sakaguchi Y, Mizui M, Kaimori JY, Takabatake Y, Isaka Y, et al.
Scientific reports (2021) 111: 73. 214 004 IHC-P; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Two isoforms of the vitamin D-dependent Ca-binding proteins have been described so far: calbindin D28k, also referred to as CALB 1, D-28k, and CAB 27, and calbindin D29k, also known as calretinin. These proteins are expressed in cells that have to handle a high calcium influx such as brain, bone, teeth, inner ear and others. Calbindins are believed to regulate cellular activity by suppressing or buffering intracellur calcium. In the brain calbindin D28k is a useful marker for specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.
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