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Home > Products > 213 211

Tyrosine hydroxylase antibody - 213 211

Tyrosine hydroxylase is the rate limiting enzyme in catecholamine synthesis
Mouse monoclonal purified IgG
Cat. No.: 213 211
Amount: 100 µg
Price: $415.00
Cat. No. 213 211 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 1000 up to 1 : 10000 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone 103E1
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to residues near the amino-terminus of rat TyrH. (UniProt Id: P04177)
Epitop AA 65 to 163 from rat TyrH (UniProt Id: P04177)
Reactivity Reacts with: rat (P04177), mouse (P24529).
Other species not tested yet.
Remarks

WB: This antibody is less sensitive than the polyclonal antibodies (cat. no. 213 102 and 213 104.
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) can be applied to improve signal quality.
Data sheet 213_211.pdf

References for Tyrosine hydroxylase - 213 211

Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 213 211 IHC; tested species: mouse
Cat. No.: 213 211
Amount: 100 µg
Price: $415.00
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 213 211 IHC; tested species: mouse
WB
ICC
ICC
IHC
IHC
IHC
IHC-P
Background
Tyrosine hydroxylase is one of the key enzymes in the synthesis pathway of catecholamines like adrenalin, noradrenalin and dopamin and is frequently used as a marker for dopaminergic neurons. This neuronal subpopulation is especially affected in Parkinson's disease.
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