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GluN1 antibody - 114 003

GluN1 is the major subunit of ionotropic NMDA type glutamate receptors
Rabbit polyclonal purified antibody
Cat. No.: 114 003
Amount: 50 µg
Price: $375.00
Cat. No. 114 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not recommended
IHC: not recommended (see remarks)
IHC-P: not tested yet
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 35 to 53 from rat GluN1 (UniProt Id: P35439)
Reactivity Reacts with: human (Q05586), rat (P35439), mouse (P35438).
No signal: zebrafish.
Other species not tested yet.
Matching control protein/peptide 114-0P
Remarks

IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
IHC: Cat. no. 114 103, is recommended for IHC.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 114 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.

Data sheet 114_003.pdf

References for GluN1 - 114 003

Molecular Characterization of AMPA-Receptor-Containing Vesicles.
Peters JJ, Leitz J, Oses-Prieto JA, Burlingame AL, Brunger AT
Frontiers in molecular neuroscience (2021) 14: 754631. 114 003 WB; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 114 003 WB; tested species: mouse
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 114 003 ELISA
Cat. No.: 114 003
Amount: 50 µg
Price: $375.00
Molecular Characterization of AMPA-Receptor-Containing Vesicles.
Peters JJ, Leitz J, Oses-Prieto JA, Burlingame AL, Brunger AT
Frontiers in molecular neuroscience (2021) 14: 754631. 114 003 WB; tested species: mouse
Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 114 003 WB; tested species: mouse
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 114 003 ELISA
Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN 1 and GluN 2 A-D. GluN 1 is the most important as it is required for activity. NMDA-receptors allow Ca2+ influx and are thought to trigger Ca2+ dependent postsynaptic processes involved in long term potentiation and depression.