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Home > Products > 110 043

Syntaxin4 antibody - 110 043 K.D.

Syntaxin4 is an ubiquitous plasma membrane protein involved in fusion of vesicles with the plasma membrane
Rabbit polyclonal purified antibody
Cat. No.: 110 043
Amount: 50 µg
Price: $455.00
Cat. No. 110 043 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: not tested yet
IHC-P: not tested yet
EM: yes

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen Recombinant protein corresponding to AA 1 to 273 from rat Syntaxin4 (UniProt Id: Q08850)
Reactivity Reacts with: human (Q12846), rat (Q08850), mouse (P70452), hamster.
Other species not tested yet.
Specificity K.D. validated PubMed: 31128202
Matching control protein/peptide 110-4P
Data sheet 110_043.pdf

References for Syntaxin4 - 110 043

Microglia modulate gliotransmission through the regulation of VAMP2 proteins in astrocytes.
Takata-Tsuji F, Chounlamountri N, Do LD, Philippot C, Novion Ducassou J, Couté Y, Ben Achour S, Honnorat J, Place C, Pascual O
Glia (2021) 691: 61-72. 110 043 WB; tested species: mouse
Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 043 WB, IP, ICC; tested species: mouse
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 110 043 WB; tested species: human,mouse
Role of SNAREs in the Atopic Dermatitis-related Cytokine Secretion and Skin-Nerve Communication.
Meng J, Wang J, Buddenkotte J, Buhl T, Steinhoff M
The Journal of investigative dermatology (2019) : . 110 043 WB; KD verified; tested species: human
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 043 WB, EM
Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth.
Ulloa F, Gonzàlez-Juncà A, Meffre D, Barrecheguren PJ, Martínez-Mármol R, Pazos I, Olivé N, Cotrufo T, Seoane J, Soriano E
PloS one (2015) 103: e0119707. 110 043 WB
The major myelin-resident protein PLP is transported to myelin membranes via a transcytotic mechanism: involvement of sulfatide.
Baron W, Ozgen H, Klunder B, de Jonge JC, Nomden A, Plat A, Trifilieff E, de Vries H, Hoekstra D
Molecular and cellular biology (2015) 351: 288-302. 110 043 WB
S-acylation of the Insulin-Responsive Aminopeptidase (IRAP): Quantitative analysis and Identification of Modified Cysteines.
Werno MW, Chamberlain LH
Scientific reports (2015) 5: 12413. 110 043 WB
Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 043 WB, IP, ICC; tested species: mouse
Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 043 WB, IP, ICC; tested species: mouse
Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.
Christensen IB, Mogensen EN, Damkier HH, Praetorius J
American journal of physiology. Cell physiology (2018) 3145: C519-C533. 110 043 IHC-P; tested species: mouse
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 043 WB, EM
Cat. No.: 110 043
Amount: 50 µg
Price: $455.00
Microglia modulate gliotransmission through the regulation of VAMP2 proteins in astrocytes.
Takata-Tsuji F, Chounlamountri N, Do LD, Philippot C, Novion Ducassou J, Couté Y, Ben Achour S, Honnorat J, Place C, Pascual O
Glia (2021) 691: 61-72. 110 043 WB; tested species: mouse
Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 043 WB, IP, ICC; tested species: mouse
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 110 043 WB; tested species: human,mouse
Role of SNAREs in the Atopic Dermatitis-related Cytokine Secretion and Skin-Nerve Communication.
Meng J, Wang J, Buddenkotte J, Buhl T, Steinhoff M
The Journal of investigative dermatology (2019) : . 110 043 WB; KD verified; tested species: human
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 043 WB, EM
Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth.
Ulloa F, Gonzàlez-Juncà A, Meffre D, Barrecheguren PJ, Martínez-Mármol R, Pazos I, Olivé N, Cotrufo T, Seoane J, Soriano E
PloS one (2015) 103: e0119707. 110 043 WB
The major myelin-resident protein PLP is transported to myelin membranes via a transcytotic mechanism: involvement of sulfatide.
Baron W, Ozgen H, Klunder B, de Jonge JC, Nomden A, Plat A, Trifilieff E, de Vries H, Hoekstra D
Molecular and cellular biology (2015) 351: 288-302. 110 043 WB
S-acylation of the Insulin-Responsive Aminopeptidase (IRAP): Quantitative analysis and Identification of Modified Cysteines.
Werno MW, Chamberlain LH
Scientific reports (2015) 5: 12413. 110 043 WB
Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 043 WB, IP, ICC; tested species: mouse
Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 043 WB, IP, ICC; tested species: mouse
Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.
Christensen IB, Mogensen EN, Damkier HH, Praetorius J
American journal of physiology. Cell physiology (2018) 3145: C519-C533. 110 043 IHC-P; tested species: mouse
Syntaxin 4 is concentrated on plasma membrane of astrocytes.
Tao-Cheng JH, Pham A, Yang Y, Winters CA, Gallant PE, Reese TS
Neuroscience (2015) 286: 264-71. 110 043 WB, EM
WB
ICC
Background

Syntaxin 4, a member of the SNARE family of proteins, is related to syntaxin 1. Like syntaxin 2 it is predominantly localized to the plasma membrane of a wide variety of cells. Similar to syntaxins 1, 2, and 3, it appears to be involved in the fusion of transport vesicles with the plasma membrane.
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