mRuby sdAb - FluoTag-X2 - N3302-AF568-L

Single domain antibody recognizing a pH resistant fluorescent protein

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N3302-AF568-L

Amount: 200 µl

Price: $515.00

Cat. No. N3302-AF568-L	200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with AZdye 568. Fluorescence labeled antibodies conjugated to Alexa^® dyes are well suited for standard epi-fluorescence setups and confocal microscopy. AZdye^® 568: λ_ex 568 nm / λ_em 665 nm (identical to Alexa^® 568) Alexa^® 647: λ_ex 650 nm / λ_em 665 nm This product or portions thereof is manufactured under license from Life Technologies Corporation. Alexa Fluor is a registered trademark of Life Technologies Corporation. Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation. Albumin was added for stabilization. For reconstitution add 200 µl H₂O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	ICC: yes
Label	AZdye 568, two fluorophores coupled to one FluoTag
Clone	1F8
Immunogen	Recombinant protein corresponding to AA 1 to 227 from Entacmaea quadricolor mRuby
Specificity	Recognizes most common mRuby derivatives

Cat. No.: N3302-AF568-L

Amount: 200 µl

Price: $515.00

Background

mRuby and mRuby derivatives are bright monomeric red fluorescent proteins derived from Entacmaea quadricolar. They show exceptional resistance to denaturation at pH extremes.

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

Protocols

ICC

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