Cat. No.: N3205-250ug
Amount: 250 µg
Price:
$355.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N3205-250ug |
250 µg purified antibody, lyophilized from PBS. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
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| Clone | 1E2 |
| Immunogen | recombinant mNeonGreen |
| Specificity | Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives. |
| Remarks |
This single domain antibody carries a C-terminal cystein available for conjugation. |
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mNeonGreen is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume. It is approximatly three times brighter compared to EGFP.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.