Cat. No.: N3202-AF647-L
Amount: 200 µl
Price:
$515.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N3202-AF647-L |
200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with Alexa 647.
Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy. AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568) This product or portions thereof is manufactured under license from Life Technologies Corporation. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Storage | –80°C for up to 12 month |
| Applications |
IP: N/A ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet |
| Label | Alexa 647, two fluorophores coupled to one FluoTag |
| Clone | 1E2 |
| Subtype | single domain |
| Immunogen | recombinant mNeonGreen |
| Specificity | Specific for mNeonGreen |
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mNeonGreen is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume. It is approximatly three times brighter compared to EGFP.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.