anti-Mouse IgG kappa light chain sdAb - N1205-HRP

A monovalent single domain secondary antibody specific for mouse IgG kappa LC

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1205-HRP

Amount: 200 µl

Price: $515.00

Cat. No. N1205-HRP	200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with HRP. Horseradish peroxidase - usually abbreviated as HRP, is one of the most commonly used reporter enzymes in biochemistry. It can be used with a huge panel of chromogenic and fluorescent substrates. Albumin was added for stabilization. For reconstitution add 200 µl H₂O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Label	HRP
Clone	1A23
Reactivity	Reacts with: mouse. No signal: chicken, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet.
Specificity	Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Cat. No.: N1205-HRP

Amount: 200 µl

Price: $515.00

Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

FluoTag^®-X2 anti-Mouse is a species-specific FluoTag^® directed against the κ light chain of mouse.
In FluoTag^®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag^® molecule. Therefore, the reagent targets four fluorophores to your primary mouse antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.

Protocols

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