Cat. No.: N1205-DBCO
Amount: 250 µg
Price:
$770.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N1205-DBCO |
250 µg purified antibody, lyophilized from PBS, fluorescence-labeled with DBCO.
Dibenzocyclooctyne (DBCO, ADIBO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC), a copper-free click chemistry reaction. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
FACS: yes |
| Label | DBCO |
| Clone | 1A23 |
| Reactivity |
Reacts with: mouse. No signal: chicken, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
| Specificity | Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins |
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Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the κ light chain of mouse.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary mouse antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.