Cat. No.: N0705-DBCO
Amount: 250 µg
Price:
$770.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N0705-DBCO |
250 µg purified antibody, lyophilized from PBS, fluorescence-labeled with
DBCO.
Albumin was added for stabilization. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
FACS: yes |
| Label | DBCO |
| Clone | 3G2 |
| Reactivity |
Reacts with: chicken. No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human. Other species not tested yet. |
| Specificity | Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins |
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Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.