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anti-Chicken IgY sdAb - N0705-Biotin

A monovalent single domain secondary antibody specific for chicken IgY

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody
Cat. No.: N0705-Biotin
Amount: 250 µg
Price: $770.00
Cat. No. N0705-Biotin 250 µg purified antibody, lyophilized from PBS, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

Albumin was added for stabilization. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
ICC: yes
FACS: yes

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.

Label biotin
Clone 3G2
Reactivity Reacts with: chicken.
No signal: mouse, rabbit, Guinea pig, donkey, rat, goat, pig, horse, cow, human.
Other species not tested yet.
Specificity Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins
Cat. No.: N0705-Biotin
Amount: 250 µg
Price: $770.00
Background

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

IgY is the major immunoglobulin found in chicken eggs. FluoTag®-X2 anti-chicken is a species-specific FluoTag®-X2 directed against immunoglobulin IgY from chicken.
In FluoTag®-X2, two fluorophore molecules are coupled site-specifically to one individual FluoTag® molecule. Therefore, the reagent targets four fluorophores to your primary chicken antibody.
Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization.