Cat. No.: N0505-250ug
Amount: 250 µg
Price:
$355.00
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This product was developed by |
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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
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| Cat. No. N0505-250ug |
250 µg purified antibody, lyophilized from PBS. For reconstitution add 250 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
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| Clone | 1H7 |
| Immunogen | Recombinant protein corresponding to AA 1 to 232 from mTag-BFP |
| Specificity | mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 |
| Remarks |
This single domain antibody carries a C-terminal cystein available for conjugation. |
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Blue fluorescent protein BFP is a monomeric fast maturating and highly photostable protein that is suited for protein labeling in acidic environments due to its high pH stability. BFP has a bright blue fluorescence with excitation/emission maxima at 402 and 457 nm and is a preferred candidate for multi color imaging.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
In FluoTag®-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets up to four fluorophores (in X4 variants) to the protein of interest, which ensures extra-bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.