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Cat. No. HS-501 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 2000 gallery |
Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of mouse CLEC4F (UniProt Id: P70194) |
Reactivity |
Reacts with: mouse (P70194). Other species not tested yet. |
Remarks |
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is recommended for immunohistochemical staining |
Data sheet | hs-501_003.pdf |
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The murine CLEC4F is a hallmark of mouse Kupffer cells (KCs) and its expression distinguishes KCs and recruited macrophages (1). CLEC4F is a C-type lectin participating in the antigen presentation of glycolipid antigens, as well as in the recognition and scavenging of desialylated platelets (2). The human CLEC4F gene does not encode a functional receptor due to a mutation in the splice acceptor site of the final exon preventing the appropriate splicing and a missense mutation disrupts the sugar-binding site (3). CLEC4F colocalises with F4/80. Although F4/80 is constitutively expressed in peripheral and resident macrophages, CLEC4F is inducible in the liver and its expression appears when primitive macrophages migrate into the fetal liver. Moreover, CLEC4F and F4/80 cells in the liver increase after infection (4).