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Cat. No. HS-477 017BT |
100 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 1000 gallery |
Label | biotin |
Clone | SY-17F7 |
Subtype | IgG2a (κ light chain) |
Immunogen | Synthetic peptide corresponding to residues near the amino terminus of mouse Galectin-3 (UniProt Id: P16110) |
Reactivity |
Reacts with: human ( P05089). Weaker signal: mouse (Q61176). Other species not tested yet. |
Data sheet | hs-477_017bt.pdf |
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Galectin-3 (Gal-3, Mac-2) is a β-galactoside binding protein that is predominantly located in the cytoplasm and shuttles into the nucleus. In addition, it is secreted to the cell surface and into biological fluids. Depending on its localization, it exerts pleiotropic biological functions like cell growth, cell differentiation, inflammation, fibrogenesis, migration, apoptosis and host defense (1). It is also involved in cancer pathogenesis, proliferation and spreading of metastasis (2). Galectin-3 is highly expressed in the covering epithelia of the digestive tract and the urinary system (3) and plays important roles in the organization of renal and intestinal cells. Furthermore, Galectin-3 has been identified in a variety of other cell types including fibroblasts, keratinocytes, granulocytes, dendritic cells and macrophages (2). Galectin-3 is expressed by certain tissue-resident macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and alveolar macrophages in the lung (3). In the brain, Galectin-3 is abundant in activated microglia but not expressed in resting cells. Following brain injury, Galectin-3 triggers anti-inflammatory properties of microglia (4). In Alzheimer’s disease, Gal-3 expression is strictly associated with microglial cell activation around Aβ plaques. In stroke Gal-3 was found to be expressed primarily in proinflammatory microglial cells (5).