Synaptic Systems

Cat. No.: HS-442 008

Amount: 50 µg

Price: $420.00

WB

IHC

IHC-P

Background

Chitinase-like protein 3 (Chil3), commonly referred to as YM1, is a rodent-specific secreted glycoprotein that belongs to the chitinase-like protein (CLP) family, sharing structural similarity with true chitinases but lacking enzymatic activity. It is predominantly expressed by alternatively activated (M2) macrophages, eosinophils, and neutrophils in mice, particularly under type 2 immune conditions such as allergic inflammation, parasitic infection, and tissue repair (1). Chil3 / YM1 expression is strongly induced by IL-4 and IL-13, hallmark cytokines of Th2-driven responses. Alongside Arginase-1 (Arg1) and Fizz1 (Retnla), Chil3 / YM1 is commonly used as a biomarker of M2 macrophage polarization in murine models (2). In healthy adult mice, Chil3 / YM1 is constitutively expressed in alveolar macrophages and neutrophils in the lung and in immature neutrophils in the spleen and bone marrow (1). Its isotype Chil4 / YM2 shows a different expression pattern and is mainly expressed in the stomach (3). In the central nervous system microglia can also induce Chil3 / YM1 expression under specific inflammatory conditions, e.g. after stroke (4).

1 ) Kang Q et al., Front Immunol (2022)
2 ) Gordon S et al., Immunity (2010)
3 ) Nio J et al., Histochem. Cell Biol. (2004)
4 ) Collmann FM et al., Front Immunol (2019)

Other products for this target

Cat. No. HS-442 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 5000 gallery IHC-P: 1 : 5000 up to 1 : 10000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb301F10
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 280 of mouse Chil3 (UniProt Id: O35744)
Reactivity	Reacts with: mouse (O35744). No signal: human. Other species not tested yet.
Specificity	Specific for mouse Chil3/YM1; no cross-reactivity with Chil4/YM2
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-301F10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	hs-442_008.pdf

Chil3 (YM1) antibody mouse specific - HS-442 008