Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

IBA1 antibody - HS-234 017

IBA1 is a marker for macrophages and microglia
Rat monoclonal purified IgG
Cat. No.: HS-234 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-234 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 200 gallery  
IHC: 1 : 100 up to 1 : 200 gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  
Clone 134G9
Subtype IgG2b (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1
Data sheet hs-234_017.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

References for IBA1 - HS-234 017

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-234 017 IHC, IHC-P; tested species: mouse
Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-234 017 IHC, IHC-P; tested species: mouse
Cat. No.: HS-234 017
Amount: 200 µl
Price: $415.00
Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-234 017 IHC, IHC-P; tested species: mouse
Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-234 017 IHC, IHC-P; tested species: mouse
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).