Cat. No.: AG-A1010
Amount: 10 ml
Price:
$280.00
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| Cat. No. AG-A1010 |
10ml 50% slurry |
| Storage | Store at 2-8 °C, do not freeze |
| Applications |
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Capacity (DBC 10%) (determined with 1 mg/ml hu IgG1 MAB using a 1 ml column). |
≥70 mg hu IgG/ml resin at residence time 6 min |
| Flow rate |
250 cm/h (50% suspension) |
| CIP (0.5 M NaOH 100 Cycles) |
No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature |
| Particle size |
45 µm |
| Formulation | 50 % slurry in PBS containing 1mM EDTA, 0.01% azide. |
| Shelf life | Stable for 6 months. |
| Data sheet | ag-a1010.pdf |
| Documents | MSDS.pdf |
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As the developer of high quality antibodies, we have extended our product portfolio by Protein A Agarose affinity resins.
Protein A is a cell wall protein from Staphylococcus aureus and is commonly used for the affinity purification or pull downs of IgG class antibodies. It binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region. Antibodies from serum (polyclonal antibodies), ascites or cell culture supernatant of hybridoma cell lines (monoclonal antibodies) can thus be easily isolated.
Protein A does not bind to all IgG classes from all species equivalently well.
| Species | Subclass | Protein A binding |
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| Human | IgA | variable |
| IgD | - | |
| IgE | - | |
| IgG1 | ++++ | |
| IgG2 | ++++ | |
| IgG3 | - | |
| IgG4 | ++++ | |
| IgM | variable | |
| Avian egg yolk | IgY | - |
| Cow | IgG | ++ |
| Dog | IgG | ++ |
| Goat | IgG | - |
| Guinea pig | IgG1 | ++++ |
| IgG2 | ++++ | |
| Hamster | IgG | + |
| Horse | IgG | ++ |
| Llama | IgG | - |
| Monkey (rhesus) | IgG | ++++ |
| Mouse | IgG1 | + |
| IgG2a | ++++ | |
| IgG2b | +++ | |
| IgG3 | ++ | |
| IgM | - | |
| Pig | IgG | +++ |
| Rabbit | IgG1 | ++++ |
| Rat | IgG1 | - |
| IgG2a | - | |
| IgG2b | - | |
| IgG3 | + | |
| Sheep | IgG | +/- |