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Home > Products > 710 006AT488

mCLING - 710 006AT488

mCling is a fixable membrane probe for high resolution microscopy
Cat. No.: 710 006AT488
Amount: 5 nmol
Price: $365.00
Cat. No. 710 006AT488

5nmol mCLING labeled with ATTO® 488 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.

 
Applications
 
ICC: 1 : 50 up to 1 : 250 (1 - 0.2 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (2 - 1 nmol/ml)

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Label ATTO 488
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.
Data sheet 710_006at488.pdf

References for mCLING - 710 006AT488

A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT488 ICC, FACS
HIV-1 Capsid Uncoating Is a Multistep Process That Proceeds through Defect Formation Followed by Disassembly of the Capsid Lattice.
Gifford LB, Melikyan GB
ACS nano (2024) 184: 2928-2947. 710 006AT488 ICC; tested species: human
Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).
Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW
eLife (2022) 11: . 710 006AT488 ICC; tested species: human
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.
Shin W, Ge L, Arpino G, Villarreal SA, Hamid E, Liu H, Zhao WD, Wen PJ, Chiang HC, Wu LG
Cell (2018) : . 710 006AT488 ICC
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT488 ICC, FACS
Preformed Ω-profile closure and kiss-and-run mediate endocytosis and diverse endocytic modes in neuroendocrine chromaffin cells.
Shin W, Wei L, Arpino G, Ge L, Guo X, Chan CY, Hamid E, Shupliakov O, Bleck CKE, Wu LG
Neuron (2021) 10919: 3119-3134.e5. 710 006AT488 UPTAKE; tested species: cow
Truncation of the otoferlin transmembrane domain alters the development of hair cells and reduces membrane docking.
Manchanda A, Bonventre JA, Bugel SM, Chatterjee P, Tanguay R, Johnson CP
Molecular biology of the cell (2021) : mbcE20100657. 710 006AT488 UPTAKE; tested species: zebrafish
Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish.
Manchanda A, Chatterjee P, Bonventre JA, Haggard DE, Kindt KS, Tanguay RL, Johnson CP
Scientific reports (2019) 91: 14273. 710 006AT488 UPTAKE; tested species: zebrafish
Cat. No.: 710 006AT488
Amount: 5 nmol
Price: $365.00
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT488 ICC, FACS
HIV-1 Capsid Uncoating Is a Multistep Process That Proceeds through Defect Formation Followed by Disassembly of the Capsid Lattice.
Gifford LB, Melikyan GB
ACS nano (2024) 184: 2928-2947. 710 006AT488 ICC; tested species: human
Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).
Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW
eLife (2022) 11: . 710 006AT488 ICC; tested species: human
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.
Shin W, Ge L, Arpino G, Villarreal SA, Hamid E, Liu H, Zhao WD, Wen PJ, Chiang HC, Wu LG
Cell (2018) : . 710 006AT488 ICC
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT488 ICC, FACS
Preformed Ω-profile closure and kiss-and-run mediate endocytosis and diverse endocytic modes in neuroendocrine chromaffin cells.
Shin W, Wei L, Arpino G, Ge L, Guo X, Chan CY, Hamid E, Shupliakov O, Bleck CKE, Wu LG
Neuron (2021) 10919: 3119-3134.e5. 710 006AT488 UPTAKE; tested species: cow
Truncation of the otoferlin transmembrane domain alters the development of hair cells and reduces membrane docking.
Manchanda A, Bonventre JA, Bugel SM, Chatterjee P, Tanguay R, Johnson CP
Molecular biology of the cell (2021) : mbcE20100657. 710 006AT488 UPTAKE; tested species: zebrafish
Otoferlin Depletion Results in Abnormal Synaptic Ribbons and Altered Intracellular Calcium Levels in Zebrafish.
Manchanda A, Chatterjee P, Bonventre JA, Haggard DE, Kindt KS, Tanguay RL, Johnson CP
Scientific reports (2019) 91: 14273. 710 006AT488 UPTAKE; tested species: zebrafish
ICC
Background
The membrane-binding fluorophore-cysteine-lysine-palmtoyl group (mCLING) is a new probe that selectively binds to the plasma membrane. It is taken up during endocytosis and, in contrast to conventional membrane dyes, remains attached to membranes after fixation and permeabilization and can therefore be combined with immunostaining and super-resolution microscopy.
mCLING was used so far in mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue.
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