Cat. No.: 534 005
Amount: 50 µg
Price:
$465.00
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| Cat. No. 534 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to the N-terminal part of mouse Reelin. (UniProt Id: Q60841) |
| Reactivity |
Reacts with: mouse (Q60841), rat (P58751). Other species not tested yet. |
| Remarks |
WB: Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. |
| Data sheet | 534_005.pdf |
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Reelin is a large secreted extracellular matrix glycoprotein. Its structure includes eight EGF-like repeats with two cleavage sites between the repeats. The central cleavage fragment is the active one as it contains the receptor binding domain (1-4).
Reelins scientific history begins in 1951 with the report of a mouse exhibiting an reeling gait (5), later found to carry a spontaneous mutation in the Reln gene. The most striking phenotype of the reeler mouse is the abnormal layering of neurons in the brain.
During development, Reelin regulates neuronal migration and proper cortical layer formation by guiding neurons to their correct positions (1,3,6). It is predominantly expressed by Cajal–Retzius cells in the hippocampus and cortex and its expression is highest during embryonic and early postnatal stages. After birth, as Cajal–Retzius cells decline, a subset of inhibitory interneurons begins to express Reelin (1-4). In the adult brain, Reelin modulates synaptic plasticity and neuroblast migration as well as dendritic spine formation (1-3).
Reelin binds to membrane receptors, including ApoER2 and VLDLR, initiating a signaling cascade via the adaptor protein Dab1, which is crucial for cytoskeletal stability and neuron positioning (1-3).
Altered Reelin expression has been associated with a variety of brain disorders including lissencephaly, Alzheimer’s disease, epilepsy, autism spectrum disorder, and schizophrenia (1,2,6,7).
In addition to the brain, Reelin is also expressed in various non-neuronal tissues and has been suggested to be implicated in processes involving the immune system, liver fibrosis and several cancers (3,4).