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S1PR1 antibody - 533 005

Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-protein-coupled receptor for sphingosine-1-phosphate
Guinea pig polyclonal purified antibody
Cat. No.: 533 005
Amount: 50 µg
Price: $465.00
Cat. No. 533 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC-Fr: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to residues near the carboxy terminus of human S1PR1 (UniProt Id: P21453)
Remarks

IHC-P: Not recommended for mouse or rat tissue. No specific signal has been observed using our standard IHC-P protocol.
IHC-Fr: The following fixatives are possible: methanol-acetone, 4% formaldehyde/PFA, methanol

Data sheet 533_005.pdf
Cat. No.: 533 005
Amount: 50 µg
Price: $465.00
Background

S1PR1, also known as S1P1 and Edg-1, is a widely expressed G-protein–coupled receptor for sphingosine-1-phosphate (S1P), a bioactive lipid found in the bloodstream. Activation of S1PR1 inhibits angiogenic sprouting and enhances cell-to-cell adhesion by regulating VE-cadherin at endothelial junctions during embryogenesis. Consequently, S1PR1 signalling plays a crucial role in vascular development and stability (1).
In adult vertebrates, S1PR1 regulates diverse physiological processes, including vascular and lymphatic permeability, astrocyte proliferation, neuronal protection, lymphocyte egress, marginal B-cell migration in secondary lymphoid organs, heart rate regulation, endothelial integrity, and responses to ischemia-reperfusion injury in multiple tissues (2,3).
S1PR1 is expressed in astrocytes and endothelial cells in the brain, where it is linked to reactive astrocytes and neuroinflammation. Recent findings show that neuronal contact induces S1PR1 expression in perisynaptic astrocyte processes, promoting astrocyte morphology, morphogenesis, and the release of synaptogenic factors crucial for neural circuit formation (4,5).