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Neurogenin2 antibody - 530 205

Neurogenin2 is a transcription factor involved in the development of the nervous system
Guinea pig polyclonal purified antibody
Cat. No.: 530 205
Amount: 50 µg
Price: $465.00
Cat. No. 530 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein comprising residues near the amino and carboxy terminus of mouse Neurogenin2 (UniProt Id: P70447)
Reactivity Reacts with: mouse (P70447).
Other species not tested yet.
Data sheet 530_205.pdf
Cat. No.: 530 205
Amount: 50 µg
Price: $465.00
IHC
IHC
IHC
Background

Neurogenins (Ngns) are basic helix-loop-helix (bHLH) transcription factors that play a crucial role in the development of the nervous system and the endocrine pancreas (1, 2). In mammals, the family comprises three members: Neurogenin1 (Ngn1), Neurogenin2 (Ngn2), and Neurogenin3 (Ngn3). Ngn1 and 2 are involved in early stages of neuronal differentiation and promote the transition of neural progenitor cells into neurons and inhibit glial cell formation in a spatially and temporally regulated manner (1, 3).
Additionally, Ngn2 plays a pivotal role in the specification of motor neurons and interneurons in the spinal cord. Phosphorylation of Ngn2 influences its ability to direct the fate of neural progenitors, affecting the balance between different neuronal subtypes (4, 5).
Ngn3 is a pro-endocrine transcription factor, primarily associated with the development of the endocrine pancreas. It is essential for the differentiation of endocrine progenitor cells into various hormone-producing cell types within the islets of Langerhans (2).