Synaptic Systems

Cat. No.: 529 004

Amount: 100 µl

Price: $370.00

ICC

IHC

Background

Corticotropin-releasing factor (CRF), also known as corticotropin-releasing hormone (CRH), is a 41-amino acid neuropeptide belonging to the corticotropin-releasing factor family. In mammals, CRF is predominantly expressed in the paraventricular nucleus of the hypothalamus, the central nucleus of the amygdala, the bed nucleus of stria terminalis (BNST), and some other brain regions (1-4).
CRF is a critical mediator of the endocrine stress response. Following stress exposure, CRF activates the hypothalamic-pituitary-adrenal (HPA) axis by stimulating the release of adrenocorticotropic hormone (ACTH) from the anterior pituitary, leading to glucocorticoid secretion from the adrenal cortex (5). Beyond its role in stress response, CRF is involved in different aspects of social behavior (4).
CRF dysregulation is implicated in stress-related mood disorders such as anxiety, depression (6, 7), and Alcohol Use Disorder (AUD) (2).

1 ) Vuppaladhadiam L et al., Cells (2020)
2 ) Snyder AE et al., Neuropharmacology (2021)
3 ) Olivares-Barraza R et al., Front Mol Neurosci (2022)
4 ) Bagosi Z et al., Biomedicines (2023)
5 ) Deussing JM et al., Physiol Rev (2018)
6 ) Maita I et al., Front Behav Neurosci (2022)
7 ) Hupalo S et al., Neurosci Biobehav Rev (2019)

Protocols

ICC

IHC

Cat. No. 529 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to the C-terminal part of the processed mouse CRF neuropeptide (UniProt Id: Q8CIT0)
Reactivity	Reacts with: mouse (Q8CIT0), rat (P01143). Other species not tested yet.
Remarks	IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet	529_004.pdf

CRF antibody - 529 004