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Home > Products > 486 003

Myosin-11 antibody - 486 003

Myosin-11 is a member of the myosin heavy chain family
Rabbit polyclonal purified antibody
Cat. No.: 486 003
Amount: 50 µg
Price: $380.00
Cat. No. 486 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 4000 (AP-staining) gallery  
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000 (see remarks)
IHC-P: 1 : 1000 up to 1 : 2000 gallery  
IHC-Fr: 1 : 2000 (see remarks) gallery  
IHC-G: 1 : 500 up to 1 : 1000

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Immunogen Recombinant protein corresponding to residues near the carboxy terminus from mouse Myosin-11. (UniProt Id: O08638)
Reactivity Reacts with: human (P35749), mouse (O08638), rat (Q63862).
Other species not tested yet.
Specificity Specific for Myosin-11, detect only isoform1.
Remarks

IHC: Heat-mediated antigen retrieval (Tris-EDTA buffer pH 9.0) is required for immunohistochemical staining.
IHC-Fr: methanol fixation is recommended
Data sheet 486_003.pdf
Cat. No.: 486 003
Amount: 50 µg
Price: $380.00
WB
IHC-P
IHC-P
IHC-P
IHC-Fr
IHC-Fr
IHC-Fr
Background

Myosin-11 also called smooth muscle myosin heavy chain or SMMHC is a myosin expressed in smooth muscle that functions as a primary contractile protein. Myosin-11 is a common myoepithelial marker in immunohistochemistry, showing cytoplasmic and membranous positivity in myoepithelial and smooth muscle cells. It tends to provide more sensitive results with less myofibroblast cross-reactivity when compared to biomarkers like calponin and smooth muscle actin (1). Four Myosin-11 isoforms exist as a result of alternative splicing of the mRNA product of a single gene (2- 5).