Synaptic Systems

Cat. No.: 480 004

Amount: 100 µl

Price: $370.00

Cat. No. 480 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	full length human recombinant PCP4 (UniProt Id: P48539)
Reactivity	Reacts with: mouse (P63054), rat (P63055). Other species not tested yet.
Data sheet	480_004.pdf

References for PCP4 - 480 004

MCU expression in hippocampal CA2 neurons modulates dendritic mitochondrial morphology and synaptic plasticity.
Pannoni KE, Fischer QS, Tarannum R, Cawley ML, Alsalman MM, Acosta N, Ezigbo C, Gil DV, Campbell LA, Farris S
Scientific reports (2025) 151: 4540. 480 004 IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 480 004 IHC; tested species: mouse

Identification of hippocampal area CA2 in hamster and vole brain.
Siegler PN, Shaughnessy EK, Horman B, Vierling TT, King DH, Patisaul HB, Huhman KL, Alexander GM, Dudek SM
The Journal of comparative neurology (2024) 5323: e25603. 480 004 IHC; tested species: mouse

Defining hippocampal area CA2 in the fox (Vulpes vulpes) brain.
Dudek SM, Phoenix AN, Scappini E, Shepeleva DV, Herbeck YE, Trut LN, Farris S, Kukekova AV
Hippocampus (2023) 336: 700-711. 480 004 IHC

Cat. No.: 480 004

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-P

Background

Purkinje-cell protein 4 (PCP4) also known as PEP-19, is a small calmodulin (CaM)-binding protein that exhibits high expression levels in cerebellar Purkinje cells. PCP4 also occurs in neocortical structures, especially in the deep layers, and in other cortical structures and parts of the hippocampus. Furthermore, the olfactory bulb and caudate putamen show high PCP4 levels. (1). PCP4 selectively binds to the C-domain of CaM via an IQ motif (2) and modulates CaM signaling by acting at the level of Ca2+ binding to CaM (3).

1 ) Renelt M et al., Acta Histochem (2014)
2 ) Wang X et al., Nat Commun (2016)
3 ) Kleerekoper QK et al., J Biol Chem (2009)

Protocols

ICC

IHC

IHC-P

PCP4 antibody - 480 004

References for PCP4 - 480 004