CaBP5 antibody - 475 002

CaBP5 is retina specific calcium binding protein

Rabbit polyclonal antiserum

Cat. No.: 475 002

Amount: 200 µl

Price: $355.00

Cat. No. 475 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 10000 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full length mouse recombinant CaBP 5 (UniProt Id: D3ZW89)
Reactivity	Reacts with: mouse (Q9JLK3), rat (D3ZW89). Other species not tested yet.
Specificity	Due to sequence homology, the antibody may exhibit some cross-reactivity to CaBP1, 2, 3 and 4.
Remarks	IHC: Brief immersion fixation of retina samples give best results. For over night fixed tissues, a heat mediated antigen retrieval (AGR) step is recommended.
Data sheet	475_002.pdf

Cat. No.: 475 002

Amount: 200 µl

Price: $355.00

IHC

Background

Calcium binding protein 5 (CaBP 5) belongs to a subfamiliy of neuronal Ca2+ binding proteins (CaBP 1-5) that show a high similarity to Calmodulin (1).
It is expressed in rod and cone bipolar cells of the retina (1) and cochlea inner hair cells of the ear (2).
CaBPs are involved in the modulation of voltage gated calcium channels, TRP channels and inositol1,4,5-triphosphate (IP3) receptors (2).
A CaBP 5 K.O. model suggests that this Ca-binding protein is involved in the rod-mediated response across the retina (3).

1 ) Haeseleer F et al., J Biol Chem (2000)
2 ) Cui G et al., J Physiol (2007)
3 ) Rieke F et al., Invest Ophthalmol Vis Sci (2008)

Protocols

IHC

Other products for this target