Synaptic Systems - P-glycoprotein1

Cat. No.: 473 005

Amount: 50 µg

Price: $460.00

WB

IHC-Fr

Background

P-glycoprotein (Pgp) is a member of the ATP-binding cassette (ABC) superfamily of transport proteins that utilize ATP to translocate a variety of lipophilic substrates across biological membranes. (1) Pgp is encoded for by a family of genes referred to as the multidrug resistance or MDR genes [2, 3] whose products function as multidrug efflux pumps [3, 4].
P-glycoprotein (Pgp) is one of the proteins expressed naturally on the plasmatic membranes of endothelial cells at the blood-brain barrier (BBB). It protects the brain from harmful substances by excluding them from entering into the parenchyma from blood circulation. It is one of several so-called efflux pumps present at the BBB and in other organs. Overexpression of this protein excludes a wide range of therapeutics for use as treatment for Central Nervous System (CNS) disorders. (5)

1 ) Higgins CF et al., Annu Rev Cell Biol (1992)
2 ) Chin KV et al., Adv Cancer Res (1993)
3 ) Gottesman MM et al., Annu Rev Biochem (1993)
4 ) Bellamy WT et al., Annu Rev Pharmacol Toxicol (1996)
5 ) Aryal M et al., PLoS One (2017)

Protocols

WB

IHC-Fr

Cat. No. 473 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP-staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: not tested yet IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse P-glycoprotein 1. (UniProt Id: P06795)
Reactivity	Reacts with: mouse (P06795), rat (P43245). Other species not tested yet.
Remarks	WB: P-glycoprotein1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC-Fr: PFA-fixation is recommended
Data sheet	473_005.pdf

P-glycoprotein1 antibody - 473 005