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Cat. No. 457 008 |
50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 1000 up to 1 : 4000 gallery |
Clone | Rb301H8 |
Subtype | IgG1 (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 52 to 143 from mouse Opalin (UniProt Id: Q7M750) |
Reactivity |
Reacts with: mouse (Q7M750), rat (Q56A26), human (Q96PE5). Other species not tested yet. |
Remarks |
This antibody is a chimeric antibody based on the monoclonal mouse antibody SY-301H8E4. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells |
Data sheet | 457_008.pdf |
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Opalin also called Tmem10 is a transmembrane protein detected specifically in mammalian oligodendrocytes. Opalin homologs are found only in mammals and not in the genome sequences of other animal classes (1). Deficient mice are born the expected Mendelian ratio and have a normal body shape and weight. No obvious abnormalities in gross anatomy or major myelin protein components nor in the compartment of myelinated axons and oligodendrocyte maturation. Nevertheless, deficient mice display increased exploratory activity in a novel environment (2).