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Home > Products > 452 005

ACTH antibody - 452 005

ACTH is a peptide hormone that stimulates the secretion of cortisol by the adrenal gland
Guinea pig polyclonal purified antibody
Cat. No.: 452 005
Amount: 50 µg
Price: $460.00
Cat. No. 452 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 134 to 162 from mouse Pro-opiomelanocortin (UniProt Id: P01193)
Reactivity Reacts with: mouse (P01193), rat (P01194).
Other species not tested yet.
Specificity The antibody is specific for ACTH. It may show minor cross-reactivity to the unprocessed precursor protein.
Remarks

IHC-P: For best results apply short antigen retrieval (10 min, citrate pH 6.0 at 97°C).
Data sheet 452_005.pdf
Cat. No.: 452 005
Amount: 50 µg
Price: $460.00
IHC
IHC
IHC
IHC
IHC-P
IHC-P
Background

Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a 39 amino acid peptide hormone. It is synthesized from the precursor POMC (pro-opiomelanocortin). POMC is proteolytically processed in a cell type specific manner to generate a couple of small biologically active peptides including ACTH (1,2).
ACTH is mainly produced and secreted by corticotroph cells of the anterior pituitary in response to corticotropin-releasing hormone from the hypothalamus. ACTH acts on the adrenal cortex of the adrenal gland to stimulate the production of glucocorticoids like cortisol. ACTH plays a pivotal role in homeostasis and stress response and is a key component of the hypothalamic-pituitary-adrenal (HPA) axis (2,3).
ACTH is also produced in extra-pituitary sites like hypothalamus and skin, where further processing of ACTH leads to the generation of alpha melanocyte-stimulating hormone (α-MSH) (2).
An excess of ACTH can cause Cushing's syndrome whereas ACTH deficiency can result in secondary adrenal insufficiency (3,4).