Synaptic Systems

Cat. No.: 417 003

Amount: 50 µg

Price: $375.00

Cat. No. 417 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 AP-staining gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 2 to 118 from mouse METTL3 (UniProt Id: Q8C3P7)
Reactivity	Reacts with: mouse (Q8C3P7), human (Q86U44), rat (F7FFC6). Other species not tested yet.
Specificity	Specific for isoform 1 K.D. validated PubMed: 34108665
Data sheet	417_003.pdf

References for METTL3 - 417 003

m6A-driven SF3B1 translation control steers splicing to direct genome integrity and leukemogenesis.
Cieśla M, Ngoc PCT, Muthukumar S, Todisco G, Madej M, Fritz H, Dimitriou M, Incarnato D, Hellström-Lindberg E, Bellodi C
Molecular cell (2023) 837: 1165-1179.e11. 417 003 WB; KD verified; tested species: human

RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability.
Xiong F, Wang R, Lee JH, Li S, Chen SF, Liao Z, Hasani LA, Nguyen PT, Zhu X, Krakowiak J, Lee DF, et al.
Cell research (2021) : . 417 003 WB; KD verified; tested species: human

Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation.
Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori EV, Shao J, Zhu X, Bolt MJ, Wu H, et al.
Molecular cell (2021) 8116: 3368-3385.e9. 417 003 WB; tested species: human

Cat. No.: 417 003

Amount: 50 µg

Price: $375.00

ICC

Background

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of RNAs at the 5'-[AG]GAC-3' consensus sites. N6-methyladenosine (m6A) has been shown to function as an important regualtory element in many processes like the circadian clock, differentiation of embryonic stem cells, primary miRNA processing, mRNA splicing, translation efficiency, mRNA editing and stability.

1 ) Batista PJ et al., Cell Stem Cell (2014)
2 ) Wang Y et al., Nat. Cell Biol. (2014)
3 ) Fustin JM et al., Cell (2013)
4 ) Lin S et al., Mol. Cell (2016)
5 ) Liu J et al., Nat. Chem. Biol. (2014)
6 ) Wang X et al., Nature (2016)

Protocols

ICC

METTL3 antibody - 417 003 K.D.

References for METTL3 - 417 003