Cat. No.: 411 017
Amount: 100 µg
Price:
$415.00
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| Cat. No. 411 017 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | SY-61A2 |
| Subtype | IgG2a (κ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208) |
| Reactivity |
Reacts with: mouse (P17208), rat (P20266). Other species not tested yet. |
| Remarks |
IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended. Alternatively, heat mediated antigen retrieval (citrate buffer pH 6) can be applied. |
| Data sheet | 411_017.pdf |
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Brn 3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.