Brn3a antibody - 411 008

Brn3a is a transcription factor involved in neuronal development

Rabbit monoclonal recombinant IgG

Cat. No.: 411 008

Amount: 50 µg

Price: $420.00

Cat. No. 411 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 400 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb61A2
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208)
Reactivity	Reacts with: mouse (P17208), rat. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-61A2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) is recommended for immunohistochemical staining.
Data sheet	411_008.pdf

Cat. No.: 411 008

Amount: 50 µg

Price: $420.00

IHC

IHC-P

Background

Brn3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.

1 ) Ghinia MG et al., J. Comp. Neurol. (2016)
2 ) Dykes IM et al., J. Neurosci. (2011)
3 ) Smith MD et al., Brain Res. Mol. Brain Res. (1999)
4 ) Xiang M et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
5 ) Budhram-Mahadeo V et al., J. Biol. Chem. (1996)
6 ) Xiang M et al., J. Neurosci. (1995)
7 ) Lillycrop KA et al., Nucleic Acids Res. (1992)

Protocols

IHC

IHC-P

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