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Brn3a antibody - 411 004

Brn3a is a transcription factor involved in neuronal development
Guinea pig polyclonal antiserum
Cat. No.: 411 004
Amount: 100 µl
Price: $365.00
Cat. No. 411 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 2000 up to 1 : 10000 (see remarks) gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208)
Reactivity Reacts with: mouse (P17208).
Other species not tested yet.
Remarks

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.

Data sheet 411_004.pdf

References for Brn3a - 411 004

Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections.
Fries M, Brown TW, Jolicoeur C, Boulan B, Boudreau-Pinsonneault C, Javed A, Abram P, Cayouette M
Cell reports (2023) 428: 112985. 411 004 IHC; tested species: mouse
Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts.
Miralles de Imperial-Ollero JA, Vidal-Villegas B, Gallego-Ortega A, Nadal-Nicolás FM, Salinas-Navarro M, Norte-Muñoz M, Di Pierdomenico J, Galindo-Romero C, Agudo-Barriuso M, Vidal-Sanz M, Valiente-Soriano FJ, et al.
Methods in molecular biology (Clifton, N.J.) (2023) 2708: 175-194. 411 004 IHC; tested species: mouse
Alpha retinal ganglion cells in pigmented mice retina: number and distribution.
Gallego-Ortega A, Norte-Muñoz M, Di Pierdomenico J, Avilés-Trigueros M, de la Villa P, Valiente-Soriano FJ, Vidal-Sanz M
Frontiers in neuroanatomy (2022) 16: 1054849. 411 004 IHC; tested species: mouse
Cat. No.: 411 004
Amount: 100 µl
Price: $365.00
Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections.
Fries M, Brown TW, Jolicoeur C, Boulan B, Boudreau-Pinsonneault C, Javed A, Abram P, Cayouette M
Cell reports (2023) 428: 112985. 411 004 IHC; tested species: mouse
Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts.
Miralles de Imperial-Ollero JA, Vidal-Villegas B, Gallego-Ortega A, Nadal-Nicolás FM, Salinas-Navarro M, Norte-Muñoz M, Di Pierdomenico J, Galindo-Romero C, Agudo-Barriuso M, Vidal-Sanz M, Valiente-Soriano FJ, et al.
Methods in molecular biology (Clifton, N.J.) (2023) 2708: 175-194. 411 004 IHC; tested species: mouse
Alpha retinal ganglion cells in pigmented mice retina: number and distribution.
Gallego-Ortega A, Norte-Muñoz M, Di Pierdomenico J, Avilés-Trigueros M, de la Villa P, Valiente-Soriano FJ, Vidal-Sanz M
Frontiers in neuroanatomy (2022) 16: 1054849. 411 004 IHC; tested species: mouse
Background

Brn 3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.