Synaptic Systems

Cat. No.: 411 004

Amount: 100 µl

Price: $365.00

Cat. No. 411 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 2000 up to 1 : 10000 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208)
Reactivity	Reacts with: mouse (P17208). Other species not tested yet.
Remarks	IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet	411_004.pdf

References for Brn3a - 411 004

Pou3f1 orchestrates a gene regulatory network controlling contralateral retinogeniculate projections.
Fries M, Brown TW, Jolicoeur C, Boulan B, Boudreau-Pinsonneault C, Javed A, Abram P, Cayouette M
Cell reports (2023) 428: 112985. 411 004 IHC; tested species: mouse

Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts.
Miralles de Imperial-Ollero JA, Vidal-Villegas B, Gallego-Ortega A, Nadal-Nicolás FM, Salinas-Navarro M, Norte-Muñoz M, Di Pierdomenico J, Galindo-Romero C, Agudo-Barriuso M, Vidal-Sanz M, Valiente-Soriano FJ, et al.
Methods in molecular biology (Clifton, N.J.) (2023) 2708: 175-194. 411 004 IHC; tested species: mouse

Alpha retinal ganglion cells in pigmented mice retina: number and distribution.
Gallego-Ortega A, Norte-Muñoz M, Di Pierdomenico J, Avilés-Trigueros M, de la Villa P, Valiente-Soriano FJ, Vidal-Sanz M
Frontiers in neuroanatomy (2022) 16: 1054849. 411 004 IHC; tested species: mouse

Cat. No.: 411 004

Amount: 100 µl

Price: $365.00

IHC

IHC-P

Background

Brn 3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.

1 ) Ghinia MG et al., J. Comp. Neurol. (2016)
2 ) Dykes IM et al., J. Neurosci. (2011)
3 ) Smith MD et al., Brain Res. Mol. Brain Res. (1999)
4 ) Xiang M et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
5 ) Budhram-Mahadeo V et al., J. Biol. Chem. (1996)
6 ) Xiang M et al., J. Neurosci. (1995)
7 ) Lillycrop KA et al., Nucleic Acids Res. (1992)

Protocols

IHC

IHC-P

Other products for this target

Brn3a antibody - 411 004

References for Brn3a - 411 004