Synaptic Systems

Cat. No.: 411 003

Amount: 50 µg

Price: $380.00

Cat. No. 411 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 5000 (see remarks) gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208)
Reactivity	Reacts with: mouse (P17208). Other species not tested yet.
Remarks	IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet	411_003.pdf

References for Brn3a - 411 003

Resveratrol attenuates retinal ganglion cell loss in a mouse model of retinal ischemia reperfusion injury via multiple pathways.
Ji K, Li Z, Lei Y, Xu W, Ouyang L, He T, Xing Y
Experimental eye research (2021) 209: 108683. 411 003 WB, IHC; tested species: mouse

Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors.
Schoepf CL, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer KK, Kress M
Brain sciences (2020) 106: . 411 003 ICC; tested species: human,mouse

Transcriptional responses in a mouse model of silicone wire embolization induced acute retinal artery ischemia and reperfusion.
Wang Y, Li Y, Feng J, Wang C, Wan Y, Lv B, Li Y, Xie H, Chen T, Wang F, Li Z, et al.
eLife (2024) 13: . 411 003 IHC; tested species: mouse

Pharmacological Activation and Transgenic Overexpression of SIRT1 Attenuate Traumatic Optic Neuropathy Induced by Blunt Head Impact.
Kwok A, Chaqour B, Khan RS, Aravand P, Dine K, Ross AG, Shindler KS
Translational vision science & technology (2024) 139: 27. 411 003 IHC; tested species: mouse

Comparison of SNCG and NEFH Promoter-Driven Expression of Human SIRT1 Expression in a Mouse Model of Glaucoma.
O'Neill N, Meng M, Chaqour B, Dine K, Sarabu N, Pham JC, Shindler KS, Ross AG
Translational vision science & technology (2024) 138: 37. 411 003 IHC; tested species: mouse

A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity.
Clayton BLL, Kristell JD, Allan KC, Cohn EF, Karl M, Jerome AD, Garrison E, Maeno-Hikichi Y, Sturno AM, Kerr A, Shick HE, et al.
Nature neuroscience (2024) 274: 656-665. 411 003 IHC; tested species: mouse

Ameliorative effect of resveratrol on acute ocular hypertension induced retinal injury through the SIRT1/NF-κB pathway.
Ji KB, Wan W, Yang Y, He XJ, Xing YQ, Hu Z
Neuroscience letters (2024) 826: 137712. 411 003 IHC; tested species: mouse

Optimizing retinal ganglion cell nuclear staining for automated cell counting.
Lin F, Lin ST, Wang J, Geisert EE
Experimental eye research (2024) 242: 109881. 411 003 IHC; tested species: mouse

AAV2 vector optimization for retinal ganglion cell-targeted delivery of therapeutic genes.
Chaqour B, Duong TT, Yue J, Liu T, Camacho D, Dine KE, Esteve-Rudd J, Ellis S, Bennett J, Shindler KS, Ross AG, et al.
Gene therapy (2024) 313-4: 175-186. 411 003 IHC; tested species: mouse

Resveratrol Ameliorates Retinal Ischemia-Reperfusion Injury by Modulating the NLRP3 Inflammasome and Keap1/Nrf2/HO-1 Signaling Pathway.
Feng J, Ji K, Pan Y, Huang P, He T, Xing Y
Molecular neurobiology (2024) : . 411 003 IHC; tested species: mouse

Intranasal Resveratrol Nanoparticles Enhance Neuroprotection in a Model of Multiple Sclerosis.
Shamsher E, Khan RS, Davis BM, Dine K, Luong V, Cordeiro MF, Shindler KS
International journal of molecular sciences (2024) 257: . 411 003 IHC; tested species: mouse

Comparison of Brn3a and RBPMS Labeling to Assess Retinal Ganglion Cell Loss During Aging and in a Model of Optic Neuropathy.
Meng M, Chaqour B, O'Neill N, Dine K, Sarabu N, Ying GS, Shindler KS, Ross AG
Investigative ophthalmology & visual science (2024) 654: 19. 411 003 IHC; tested species: mouse

A Non-canonical Excitatory PV RGC-PV SC Visual Pathway for Mediating the Looming-evoked Innate Defensive Response.
Yuan M, Jin S, Tan G, Song S, Liu Y, Wang H, Shen Y
Neuroscience bulletin (2024) 403: 310-324. 411 003 IHC; tested species: mouse

Reduction of retinal ganglion cell death in mouse models of familial dysautonomia using AAV-mediated gene therapy and splicing modulators.
Schultz A, Cheng SY, Kirchner E, Costello S, Miettinen H, Chaverra M, King C, George L, Zhao X, Narasimhan J, Weetall M, et al.
Scientific reports (2023) 131: 18600. 411 003 IHC; tested species: mouse

Optimisation of AAV-NDI1 Significantly Enhances Its Therapeutic Value for Correcting Retinal Mitochondrial Dysfunction.
Chadderton N, Palfi A, Maloney DM, Carrigan M, Finnegan LK, Hanlon KS, Shortall C, O'Reilly M, Humphries P, Cassidy L, Kenna PF, et al.
Pharmaceutics (2023) 152: . 411 003 IHC; tested species: mouse

Inhibition of Heat Shock Protein B8 Alleviates Retinal Dysfunction and Ganglion Cells Loss Via Autophagy Suppression in Mouse Axonal Damage.
Xie F, Li Z, Yang N, Yang J, Hua D, Luo J, He T, Xing Y
Investigative ophthalmology & visual science (2022) 636: 28. 411 003 IHC; tested species: mouse

SARM1 Ablation Is Protective and Preserves Spatial Vision in an In Vivo Mouse Model of Retinal Ganglion Cell Degeneration.
Finnegan LK, Chadderton N, Kenna PF, Palfi A, Carty M, Bowie AG, Millington-Ward S, Farrar GJ
International journal of molecular sciences (2022) 233: . 411 003 IHC; tested species: mouse

Therapeutic Potential of a Novel Glucagon-like Peptide-1 Receptor Agonist, NLY01, in Experimental Autoimmune Encephalomyelitis.
Gharagozloo M, Smith MD, Sotirchos ES, Jin J, Meyers K, Taylor M, Garton T, Bannon R, Lord HN, Dawson TM, Dawson VL, et al.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2021) 183: 1834-1848. 411 003 IHC; tested species: mouse

Krüppel-like factor 7 protects retinal ganglion cells and promotes functional preservation via activating the Akt pathway after retinal ischemia-reperfusion injury.
Li Z, Xie F, Yang N, Yang J, Luo J, Hua D, He T, Xing Y
Experimental eye research (2021) 207: 108587. 411 003 IHC; tested species: mouse

Neuroprotection mediated by ST266 requires full complement of proteins secreted by amnion-derived multipotent progenitor cells.
Willett K, Khan RS, Dine K, Wessel H, Kirshner ZZ, Sauer JL, Ellis A, Brown LR, Shindler KS
PloS one (2021) 161: e0243862. 411 003 IHC; tested species: mouse

SIRT1 is required for the neuroprotection of resveratrol on retinal ganglion cells after retinal ischemia-reperfusion injury in mice.
Luo J, He T, Yang J, Yang N, Li Z, Xing Y
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (2020) 2582: 335-344. 411 003 IHC; tested species: mouse

GLP-1 Receptor Agonist NLY01 Reduces Retinal Inflammation and Neuron Death Secondary to Ocular Hypertension.
Sterling JK, Adetunji MO, Guttha S, Bargoud AR, Uyhazi KE, Ross AG, Dunaief JL, Cui QN
Cell reports (2020) 335: 108271. 411 003 IHC; tested species: mouse

Mapping of Extrinsic Innervation of the Gastrointestinal Tract in the Mouse Embryo.
Niu X, Liu L, Wang T, Chuan X, Yu Q, Du M, Gu Y, Wang L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4035: 6691-6708. 411 003 IHC; tested species: mouse

Amnion-Derived Multipotent Progenitor Cells Suppress Experimental Optic Neuritis and Myelitis.
Khan RS, Ross AG, Willett K, Dine K, Banas R, Brown LR, Shindler KS
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2020) : . 411 003 IHC; tested species: mouse

One proline deletion in the fusion peptide of neurotropic mouse hepatitis virus (MHV) restricts retrograde axonal transport and neurodegeneration.
Rout SS, Singh M, Shindler KS, Das Sarma J
The Journal of biological chemistry (2020) 29520: 6926-6935. 411 003 IHC; tested species: mouse

Oral administration of the iron chelator deferiprone protects against loss of retinal ganglion cells in a mouse model of glaucoma.
Cui QN, Bargoud AR, Ross AG, Song Y, Dunaief JL
Experimental eye research (2020) : 107961. 411 003 IHC; tested species: mouse

Cat. No.: 411 003

Amount: 50 µg

Price: $380.00

Optimizing retinal ganglion cell nuclear staining for automated cell counting.
Lin F, Lin ST, Wang J, Geisert EE
Experimental eye research (2024) 242: 109881. 411 003 IHC; tested species: mouse

IHC

IHC-P

Background

Brn 3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.

1 ) Ghinia MG et al., J. Comp. Neurol. (2016)
2 ) Dykes IM et al., J. Neurosci. (2011)
3 ) Smith MD et al., Brain Res. Mol. Brain Res. (1999)
4 ) Xiang M et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
5 ) Budhram-Mahadeo V et al., J. Biol. Chem. (1996)
6 ) Xiang M et al., J. Neurosci. (1995)
7 ) Lillycrop KA et al., Nucleic Acids Res. (1992)

Protocols

IHC

IHC-P

Other products for this target

Brn3a antibody - 411 003

References for Brn3a - 411 003