Kv1.2 antibody - 402 005

Kv1.2 is a potassium channel in the nervous system

Guinea pig polyclonal purified antibody

Cat. No.: 402 005

Amount: 50 µg

Price: $460.00

Cat. No. 402 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat Kv1.2 (UniProt Id: P63142)
Reactivity	Reacts with: rat (P63142), mouse (P63141). Other species not tested yet.
Remarks	WB: Kv1.2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	402_005.pdf

Cat. No.: 402 005

Amount: 50 µg

Price: $460.00

IHC-P

Background

A high diversity of K+ channels is formed in the nervous system by heteromeric assembly of subunits encoded by a large number of K+ channel genes. They play an important role in regulating the level of neuronal excitability.
Deletion of Kv 1.2 is lethal in mice and mutations lead to severe consequences including epileptic encephalopathy, intellectual disability and episodic ataxia in humans.

1 ) Baronas VA et al., Sci Rep (2017)
2 ) Yokoyama S et al., Ann. N. Y. Acad. Sci. (1999)
3 ) Elinder F et al., Biophys. J. (2016)
4 ) Baronas VA et al., Channels (Austin) (2016)
5 ) Hyun JH et al., J. Physiol. (Lond.) (2013)
6 ) Williams MR et al., J. Neurosci. (2012)
7 ) Jogini V et al., Biophys. J. (2007)
8 ) Chung YH et al., Brain Res. (2001)
9 ) Wang H et al., J. Neurosci. (1994)

Protocols

IHC-P

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