Synaptic Systems

Cat. No.: 390 005

Amount: 50 µg

Price: $470.00

Cat. No. 390 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 225 from sea anemone DsRed (UniProt Id: Q9U6Y8)
Specificity	Recognizes mRFP, mCherry, mOrgange2, dsRed, tdTomato, mScarlet.
Data sheet	390_005.pdf

References for RFP - 390 005

Divergent opioid-mediated suppression of inhibition between hippocampus and neocortex across species and development.
Caccavano AP, Vlachos A, McLean N, Kimmel S, Kim JH, Vargish G, Mahadevan V, Hewitt L, Rossi AM, Spineux I, Wu SJ, et al.
Neuron (2025) : . 390 005 IHC; tested species: mouse

Hippocampus-to-amygdala pathway drives the separation of remote memories of related events.
Concina G, Milano L, Renna A, Manassero E, Stabile F, Sacchetti B
Cell reports (2024) 435: 114151. 390 005 IHC; tested species: rat

Individual thalamic inhibitory interneurons are functionally specialized toward distinct visual features.
Müllner FE, Roska B
Neuron (2024) 11216: 2765-2782.e9. 390 005 IHC; tested species: mouse

Purkinje cell microzones mediate distinct kinematics of a single movement.
Blot FGC, White JJ, van Hattem A, Scotti L, Balaji V, Adolfs Y, Pasterkamp RJ, De Zeeuw CI, Schonewille M
Nature communications (2023) 141: 4358. 390 005 IHC; tested species: mouse

Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments.
Ogawa Y, Lim BC, George S, Oses-Prieto JA, Rasband JM, Eshed-Eisenbach Y, Hamdan H, Nair S, Boato F, Peles E, Burlingame AL, et al.
Nature communications (2023) 141: 6797. 390 005 IHC; tested species: rat

Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S.
Asencor AI, Dvoryanchikov G, Makhoul V, Tsoulfas P, Chaudhari N
eNeuro () 93: . 390 005 IHC; tested species: mouse

Cat. No.: 390 005

Amount: 50 µg

Price: $470.00

Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S.
Asencor AI, Dvoryanchikov G, Makhoul V, Tsoulfas P, Chaudhari N
eNeuro () 93: . 390 005 IHC; tested species: mouse

ICC

IHC

Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D et al., Genesis (2010)
2 ) Shaner NC et al., Nat. Biotechnol. (2004)
3 ) Labas YA et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
4 ) Fradkov AF et al., FEBS Lett. (2000)

Protocols

ICC

IHC

Other products for this target

RFP (dsRed) antibody - 390 005

References for RFP - 390 005