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Home > Products > 385 005

Monoamine oxidase B antibody - 385 005

Monoamine oxidase B catalyzes the oxidative deamination of monoamines
Guinea pig polyclonal purified antibody
Cat. No.: 385 005
Amount: 50 µg
Price: $460.00
Cat. No. 385 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 500      gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 455 to 464 from mouse MAOB (UniProt Id: Q8BW75)
Reactivity Reacts with: mouse (Q8BW75), rat (P19643).
Other species not tested yet.
Specificity Specific for Monoamine oxidase B.
Data sheet 385_005.pdf
Cat. No.: 385 005
Amount: 50 µg
Price: $460.00
WB
IHC
IHC-P
IHC-P
Background
Monoamine oxidases (MAOs) belong to the family of flavin-containing amine oxidoreductases and are integral proteins of outer mitochondrial membranes. Isoenzymes MAO-A and MAO-B share roughly 70% of their structure. They occur in various cells in peripheral organs and in the CNS in both neuronal and non-neuronal cells, where they oxidatively deaminate biogenic and xenobiotic amines. They play an important role in the CNS in metabolic inactivation of released monoamine transmitters (catecholamines, serotonin and trace amines) and in detoxification of xenobiotic amines.
In rodent brain MAO-B metabolizes the trace amines phenylethylamine, methylhistamine, tyramine as well as octopamine. In human brain dopamine is also a substrate. Recent results identified MAO-B as a synthesizing enzyme of glial GABA, which is released to mediate tonic inhibition in the cerebellum and striatum. MAO-B is expressed in the CNS in serotonergic as well as in histaminergic neurons and in some astrocytes. The highest levels of MAO-B in peripheral organs are found in the liver and in the islets of Langerhans.
MAO inhibitors have provided therapies for both psychiatric as well as neurologic disorders. MAO-B has been reported to increase with aging and in several regions of the CNS in association with neurodegenerative disorders such as Parkinson's, Alzheimer's and Huntington's diseases. Plaque-associated astrocytes in Alzheimer's disease cortices have been shown to contain an increased amount of MAO-B activity.