DARPP32 antibody - 382 003

DARPP32 is a dopamine and cAMP regulated phosphoprotein

Rabbit polyclonal purified antibody

Cat. No.: 382 003

Amount: 50 µg

Price: $455.00

Cat. No. 382 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 148 to 166 from mouse Darpp32 (UniProt Id: Q60829)
Reactivity	Reacts with: rat (Q60829), mouse (Q6J4I0). Other species not tested yet.
Matching control protein/peptide	382-0P
Data sheet	382_003.pdf

Cat. No.: 382 003

Amount: 50 µg

Price: $455.00

IHC

IHC-P

Background

The dopamine and cAMP regulated phosphoprotein 32 kDa (DARPP 32), also known as PPP1R1B, is phosphorylated in a dopamine dependent manner. Stimulation of the dopamine receptor DRD 1 increases cAMP levels resulting in DARPP 32 phosphorylation. It is a commonly used marker for striatal medium spiny neurons (MSNs).

1 ) Srivastava P et al., Mol. Neurobiol. (2016)
2 ) Collier TJ et al., Neurobiol. Dis. (2015)
3 ) Aubry L et al., Proc. Natl. Acad. Sci. U.S.A. (2008)
4 ) Ehrman LA et al., Genes Brain Behav. (2006)
5 ) Reiner A et al., J. Chem. Neuroanat. (1998)

Protocols

IHC

IHC-P

OTHER

Preabsorbtion

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