DOPA decarboxylase antibody - 369 004

DOPA decarboxylase is an enzyme for dopamine, serotonin, and trace amine biosynthesis.

Guinea pig polyclonal antiserum

Cat. No.: 369 004

Amount: 100 µl

Price: $365.00

Cat. No. 369 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 480 from mouse DDC (UniProt Id: O88533)
Reactivity	Reacts with: mouse (O88533), rat (P14173). Other species not tested yet.
Specificity	DOPA decarboxylase
Data sheet	369_004.pdf

Cat. No.: 369 004

Amount: 100 µl

Price: $365.00

IHC

Background

DOPA decarboxylase, also referred to as aromatic L-amino acid decarboxylase, AADC or DDC, is the second enzyme in the biosynthesis of dopamine and serotonin. In addition, it is responsible for the synthesis of the trace amines phenylethylamine, p-tyramine, and tryptamine, which are considered to act as neuromodulators.
DDC is expressed in the central nervous system, but has also been detected in several tissues such as liver, kidney, small intestine, adrenal gland and blood vessels.
The inhibition of DDC is used for the treatment of Parkinson's disease.

1 ) Haycock JW et al., J. Neurochem. (2003)
2 ) Burkhard P et al., Nat. Struct. Biol. (2001)
3 ) Berry MD et al., Neurochem. Res. (1996)
4 ) Ishii S et al., J. Biochem. (1996)
5 ) Albert VR et al., Proc. Natl. Acad. Sci. U.S.A. (1992)
6 ) Jaeger CB et al., Neuroscience (1984)

Protocols

IHC

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