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Home > Products > Last filter > 363 003

N-Cadherin antibody - 363 003

N-Cadherin is a cell adhesion protein
Rabbit polyclonal purified antibody
Cat. No.: 363 003
Amount: 50 µg
Price: $380.00
Cat. No. 363 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 746 to 906 from mouse N-Cadherin (UniProt Id: P15116)
Reactivity Reacts with: rat (Q9Z1Y3), mouse (P15116).
Other species not tested yet.
Specificity Recognizes N-cadherin. The antibody may crossreact to other cadherins due to sequence homology.
Remarks

IHC: Fix for 15 min with 4% PFA and 15% picric acid in PBS.
Data sheet 363_003.pdf

References for N-Cadherin - 363 003

Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.
Benavides-Rivas C, Tovar LM, Zúñiga N, Pinto-Borguero I, Retamal C, Yévenes GE, Moraga-Cid G, Fuentealba J, Guzmán L, Coddou C, Bascuñán-Godoy L, et al.
Frontiers in pharmacology (2020) 11: 900. 363 003 WB; tested species: frog
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 363 003 IHC; tested species: rat
Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.
Christensen IB, Mogensen EN, Damkier HH, Praetorius J
American journal of physiology. Cell physiology (2018) 3145: C519-C533. 363 003 IHC-P; tested species: mouse
Cat. No.: 363 003
Amount: 50 µg
Price: $380.00
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.
Benavides-Rivas C, Tovar LM, Zúñiga N, Pinto-Borguero I, Retamal C, Yévenes GE, Moraga-Cid G, Fuentealba J, Guzmán L, Coddou C, Bascuñán-Godoy L, et al.
Frontiers in pharmacology (2020) 11: 900. 363 003 WB; tested species: frog
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 363 003 IHC; tested species: rat
Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.
Christensen IB, Mogensen EN, Damkier HH, Praetorius J
American journal of physiology. Cell physiology (2018) 3145: C519-C533. 363 003 IHC-P; tested species: mouse
WB
ICC
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
Background

N-Cadherin or neural cadherin also known as Cadherin 2 (CDH 2) or NCAD is a type-I classical cadherin, along with E-Cadherin, P-Cadherin, R-Cadherin, and M-Cadherin. This transmembrane protein plays a crucial role in calcium-dependent cell-cell adhesion. In an anti-parallel conformation, its extracellular region interacts with another N-Cadherin molecule on an adjacent cell, forming a linear adhesive "zipper" through repeated dimer interfaces. Its C-terminal, cytoplasmic tail binds to catenins, which in turn link to the actin cytoskeleton (1). N-Cadherin is prevalent in non-epithelial tissues and is expressed in different types of cells such as neural cells, endothelial cells, stromal cells and osteoblasts. It plays an important role in renal epithelial integrity and polarity and is expressed in proximal renal tubules (2). Additionally, N-Cadherin plays a key role in reproductive biology (3) and in myocardium (4). In neural tissue, N-Cadherin replaces E-Cadherin during neurulation, forming strong adherens junctions to maintain tissue architecture and regulates proliferation and differentiation of neural progenitor cells. Cleavage of N-Cadherin modulates adult neural stem cell functional quiescence (5).
N-Cadherin serves as an indicator of ongoing epithelial-to-mesenchymal transition (EMT) and its expression has been correlated with the development of various types of carcinomas. It also promotes angiogenesis and the integrity of blood vessels by ensheathing endothelial and mural cells, stabilizing microvessels (6).