Synaptic Systems - Monocarboxylate Transporter4

Cat. No.: 356 405

Amount: 50 µg

Price: $460.00

WB

IHC-P

Background

The proton-linked transport of monocarboxylates across the plasma membrane plays a critical role in the metabolism and pH regulation of most cells. Fourteen different monocarboxylic acid transporters (MCTs) have been identified, so far. Only four of them (MCT 1-4) have been shown to actively transport the monocarboxylates pyruvate and lactate across the cell membrane.
MCT 1, also referrred to as Slc16 a1, and MCT 2 show differential expression in astrocyte subpopulations and blood vessels in the mammalian brain.
MCT4 is highly expressed in glycolytic cells, such as skeletal muscle fibers, astrocytes, leukocytes, chondrocytes, and some mammalian cell lineages.

1 ) Morrison BM et al., Exp. Neurol. (2015)
2 ) Smith JP et al., Brain Res. (2012)
3 ) Wilson MC et al., J. Biol. Chem. (2009)
4 ) Manoharan C et al., Mol. Membr. Biol. ()
5 ) Hanu R et al., Am. J. Physiol., Cell Physiol. (2000)
6 ) Jackson VN et al., Biochim. Biophys. Acta (1995)
7 ) Chiry O et al., Brain Res. (2006)

Protocols

WB

IHC-P

Cat. No. 356 405	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 447 to 459 from rat Monocarboxylate transporter 4 (UniProt Id: O35910)
Reactivity	Reacts with: rat (O35910), human (O15427), mouse (P57787). Other species not tested yet.
Specificity	Monocarboxylate transporter 4
Data sheet	356_405.pdf

Monocarboxylate Transporter4 antibody - 356 405