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Monocarboxylate Transporter2 (MCT2, SLC16A7) antibody - 356 205

MCT2 is a neuronal lactate transporter.
Guinea pig polyclonal purified antibody
Cat. No.: 356 205
Amount: 50 µg
Price: $460.00
Cat. No. 356 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 3000 (AP-staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to residues near the carboxy region of rat Monocarboxylate transporter2. (UniProt Id: Q63344)
Reactivity Reacts with: mouse (O70451), rat (Q63344).
Other species not tested yet.
Data sheet 356_205.pdf
Cat. No.: 356 205
Amount: 50 µg
Price: $460.00
WB
Background

Monocarboxylate transporter2 (MCT2), encoded by SLC16A7, is a high-affinity transporter primarily expressed in neurons, where it facilitates lactate uptake a key component of the astrocyte-neuron lactate shuttle (ANLS). This system supports neuronal metabolism by delivering astrocyte-derived lactate to neurons, especially during synaptic activity or glucose limitation (1,2). MCT2 is localized to dendrites and postsynaptic densities and shows highest expression in the cerebellum and hippocampus (2,3). Its developmental expression parallels synaptogenesis and glucose demand (1). MCT2's low Km (~0.7 mM) makes it effective at physiological lactate levels (1). It is also expressed in hypothalamic neurons involved in feeding regulation, linking lactate sensing to metabolic status (4). Altogether, MCT2 plays a crucial role in neuronal energy metabolism and may be implicated in neurodegenerative and metabolic disorders.