Tryptophan hydroxylase2 antibody - 348 011 K.O.

Tryptophan hydroxylase2 is an enzyme involved in serotonin synthesis

Mouse monoclonal purified IgG

Cat. No.: 348 011

Amount: 100 µg

Price: $415.00

Cat. No. 348 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 100 up to 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	269E7
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 139 from rat Tryptophanhydroxylase 2 (UniProt Id: Q8CGU9)
Reactivity	Reacts with: rat (Q8CGU9), mouse (Q8CGV2). Other species not tested yet.
Specificity	K.O. validated
Data sheet	348_011.pdf

Cat. No.: 348 011

Amount: 100 µg

Price: $415.00

IHC

IHC-P

Background

Tryptophan hydroxylase (TPH) is an enzyme that catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin).
Two isoforms TPH 1 and TPH 2 have been described. TPH 1 occurs mainly in tissues that express serotonin in the periphery (skin, gut, pineal gland). TPH 2 is exclusively expressed in neuronal cell types and is the predominant isoform in the central nervous system.
In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain.

1 ) Chen GL et al., Biochem. Pharmacol. (2013)
2 ) Martínez A et al., Curr. Med. Chem. (2001)
3 ) Garber SL et al., Brain Res. (1987)

Protocols

IHC

IHC-P

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