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Home > Products > 348 008

Tryptophan hydroxylase2 antibody - 348 008 K.O.

Tryptophan hydroxylase2 is an enzyme involved in serotonin synthesis
Rabbit monoclonal recombinant IgG
Cat. No.: 348 008
Amount: 50 µg
Price: $415.00
Cat. No. 348 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 400 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone Rb269E7
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 139 from rat Tryptophanhydroxylase 2 (UniProt Id: Q8CGU9)
Reactivity Reacts with: rat (Q8CGU9), mouse (Q8CGV2).
Other species not tested yet.
Specificity K.O. validated
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 269E7. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet 348_008.pdf
Cat. No.: 348 008
Amount: 50 µg
Price: $415.00
IHC
IHC
IHC-P
Background
Tryptophan hydroxylase (TPH) is an enzyme that catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin).
Two isoforms TPH 1 and TPH 2 have been described. TPH 1 occurs mainly in tissues that express serotonin in the periphery (skin, gut, pineal gland). TPH 2 is exclusively expressed in neuronal cell types and is the predominant isoform in the central nervous system.
In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain.
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