Tryptophan hydroxylase2 antibody - 348 003 K.O.

Tryptophan hydroxylase2 is an enzyme involved in serotonin synthesis

Rabbit polyclonal purified antibody

Cat. No.: 348 003

Amount: 100 µl

Price: $375.00

Cat. No. 348 003	100 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	IHC: 1 : 250 up to 1 : 500 gallery IHC-P: not tested yet Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 139 from rat Tryptophanhydroxylase 2 (UniProt Id: Q8CGU9)
Reactivity	Reacts with: rat (Q8CGU9), mouse (Q8CGV2). Other species not tested yet.
Specificity	K.O. validated
Data sheet	348_003.pdf

Cat. No.: 348 003

Amount: 100 µl

Price: $375.00

IHC

Background

Tryptophan hydroxylase (TPH) is an enzyme that catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin).
Two isoforms TPH 1 and TPH 2 have been described. TPH 1 occurs mainly in tissues that express serotonin in the periphery (skin, gut, pineal gland). TPH 2 is exclusively expressed in neuronal cell types and is the predominant isoform in the central nervous system.
In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain.

1 ) Chen GL et al., Biochem. Pharmacol. (2013)
2 ) Martínez A et al., Curr. Med. Chem. (2001)
3 ) Garber SL et al., Brain Res. (1987)

Protocols

IHC

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