SOX2 antibody - 347 003C3

SOX proteins are transcription factors involved in CNS development

Rabbit polyclonal purified antibody

Cat. No.: 347 003C3

Amount: 50 µg

Price: $465.00

Cat. No. 347 003C3	50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Cyanine 3. Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy. Cyanine 2: λ_ex 492 nm / λ_em 508 nm Sulfo-Cyanine 3: λ_ex 554 nm / λ_em 568 nm Sulfo-Cyanine 5: λ_ex 649 nm / λ_em 667 nm (Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy. Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	Sulfo-Cyanine 3
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse SOX2 (UniProt Id: P48432)
Reactivity	Reacts with: rat (D4A543), mouse (P48432), human (P48431). Other species not tested yet.
Data sheet	347_003c3.pdf

Cat. No.: 347 003C3

Amount: 50 µg

Price: $465.00

ICC

IHC

Background

Sex determining region of Y chromosome (Sry)-related high mobility group box1-3 or SOX 1-3 proteins belong to the earliest transcription factors expressed in the developing CNS
SOX1, SOX2 and SOX3 constitute the B1-subgroup of the Sox gene family 12. They are expressed by most progenitor cells of the developing CNS and are generally downregulated by neural cells when they exit the cell cycle and differentiate.

1 ) Poh YC et al., Nat Commun (2014)
2 ) Thiel G et al., Biochem. J. (2013)
3 ) Archer TC et al., Dev. Biol. (2011)
4 ) Kan L et al., Dev. Biol. (2004)
5 ) Bylund M et al., Nat. Neurosci. (2003)
6 ) Bani-Yaghoub M et al., Dev. Biol. (2006)
7 ) Graham V et al., Neuron (2003)
8 ) Wood HB et al., Mech. Dev. (1999)
9 ) Pevny LH et al., Development (1998)
10 ) Stevanović M et al., Hum. Mol. Genet. (1993)

Protocols

ICC

IHC

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