Cat. No.: 342 403
Amount: 50 µg
Price:
$375.00
Cat. No. 342 403 | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 1000 up to 1 : 2000 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.', $event)" style="cursor: help;">ExM: 1 : 200 (see remarks) gallery |
Immunogen | Recombinant protein corresponding to AA 608 to 691 from human DAP4 (UniProt Id: Q9Y2H0) |
Reactivity |
Reacts with: rat (P97839), human (Q9Y2H0), mouse (B1AZP2). Other species not tested yet. |
Remarks |
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981) |
Data sheet | 342_403.pdf |
Disks large associated proteins (DLGAPs or DAPs) also known as SAP90/PSD-95-associated proteins (SAPAPs) are members of a protein family whose members interact with a membrane-associated guanylate kinase localized at the postsynaptic density (PSD) in neuronal cells. DAPs are adaptor proteins that also interact with other synaptic scaffolding proteins like shanks.