Synaptic Systems

Cat. No.: 340 004

Amount: 100 µl

Price: $370.00

Cat. No. 340 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 77 from mouse SERT (UniProt Id: Q60857)
Reactivity	Reacts with: rat (P31652), mouse (Q60857). Other species not tested yet.
Data sheet	340_004.pdf

References for SERT - 340 004

A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility.
Sheu SH, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, Walpita D, Pasolli HA, Houser J, Sanchez-Martinez S, Brauchi SE, et al.
Cell (2022) 18518: 3390-3407.e18. 340 004 ICC, IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 340 004 IHC; tested species: mouse

Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.
Liu X, Sun YX, Zhang CC, Zhang XQ, Zhang Y, Wang T, Ma YN, Wang H, Su YA, Li JT, Si TM, et al.
Neuropharmacology (2021) : 108468. 340 004 IHC; tested species: mouse

Regulation of dopamine neurotransmission from serotonergic neurons by ectopic expression of the dopamine D2 autoreceptor blocks levodopa-induced dyskinesia.
Sellnow RC, Newman JH, Chambers N, West AR, Steece-Collier K, Sandoval IM, Benskey MJ, Bishop C, Manfredsson FP
Acta neuropathologica communications (2019) 71: 8. 340 004 IHC; tested species: rat

Cat. No.: 340 004

Amount: 100 µl

Price: $370.00

IHC

IHC-P

Background

This serotonin transporter (SERT), also referred to as 5HTT and Slc6a4, is a monoamine transporter protein that transports serotonin back from the synaptic cleft into the presynaptic neuron to terminate the action of this neurotransmitter.

1 ) Montgomery TR et al., J. Neurosci. (2014)
2 ) Noristani HN et al., Cell Death Dis (2011)
3 ) Legutko R et al., Brain Res. (2001)
4 ) Najib A et al., FEBS Lett. (2000)
5 ) Fujita M et al., Neurosci. Lett. (1993)
6 ) Langer SZ et al., Experientia (1988)

Protocols

IHC

IHC-P

Other products for this target

SERT antibody - 340 004

References for SERT - 340 004