Synaptic Systems

Cat. No.: 338 003

Amount: 50 µg

Price: $375.00

Structural arrangement of auditory brainstem nuclei in the bats Phyllostomus discolor and Carollia perspicillata.
Pätz C, Console-Meyer L, Felmy F
The Journal of comparative neurology (2022) : . 338 003 IHC

WB

ICC

IHC

Background

Hyperpolarization-activated, cyclic nucleotide-gated channels (HCNs) are a distinct class of ion channels that are widely distributed across the mammalian brain.
HCN channels are either homomeric or heteromeric compositions of the 4 subunits HCN 1-4.

1 ) Noam Y et al., J. Biol. Chem. (2014)
2 ) Rinaldi A et al., J. Physiol. (Lond.) (2013)
3 ) Luján R et al., Eur. J. Neurosci. (2005)
4 ) Lörincz A et al., Nat. Neurosci. (2002)

Protocols

WB

ICC

IHC

Cat. No. 338 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 849 to 910 from rat HCN1 (UniProt Id: Q9JKB0)
Reactivity	Reacts with: rat (Q9JKB0), mouse (O88704). Other species not tested yet.
Data sheet	338_003.pdf

HCN1 antibody - 338 003

References for HCN1 - 338 003